BIOL 3020 Exam 3 — Study Command Center

5' cap: m7G added via 5'-5' triphosphate bond. Protects from exonucleases; required for ribosome recognition.
Splicing: Spliceosome (U1, U2, U4, U5, U6 snRNPs). Recognition: 5&prime; GU, branch point A, 3&prime; AG. Lariat intermediate.
Alternative splicing: ~75% of human genes. Different exon combinations &rarr; multiple proteins from one gene.
3' poly-A tail: AAUAAA signal &rarr; cleavage + ~200 A residues.

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